41 The neurokinin receptors are G-protein-coupled receptors with the characteristic seven-membrane-spanning domains.9 In general, several mechanisms prevent the uncontrolled Gefitinib mouse stimulation of cells by neurotransmitters that interact with G-protein-coupled receptors: (i) removal of the agonist from the extracellular fluid by degradation

or reuptake (the latter is not relevant to the tachykinins, as stated above); (ii) desensitization of the receptor by uncoupling from the G-proteins to terminate the signal transduction cascade; and (iii) endocytosis of the agonist-stimulated receptor, Inhibitors,research,lifescience,medical depleting the plasma membrane of high-affinity receptors.42 The NK1 receptor appears to be desensitized by phosphorylation, independently of receptor internalization, while resensitization requires endocytosis, recycling, and dephosphorylation.43-44 The prompt tachyphylaxis of the NK1 receptor after exposure to the

agonist is, however, linked to the rapid receptor internalization.45 Anatomic distribution of neurokinin receptors within the CNS A wide variety of brain regions express the NK1 receptor. Inhibitors,research,lifescience,medical Notably, the raphe nuclei, locus ceruleus, striatum, the nucleus accumbens, the hippocampus, the lateral nucleus of the hypothalamus, the habenula, the interpeduncular nucleus, the nucleus of the tractus solitarius, and the substantia nigra are all rich in NK1 receptors.46-48 Thus, there is a remarkable mismatch between SP-containing Inhibitors,research,lifescience,medical brain regions and NK1 receptor-expressing brain regions, which may be due to the aforementioned limited selectivity of the tachykinins. NK2 receptors are sparsely distributed in the CNS. They can be found in low amounts in various regions, Inhibitors,research,lifescience,medical such as the striatum. The third type of neurokinin receptor, the NK3 receptor, Inhibitors,research,lifescience,medical is strikingly prevalent in midcortical laminae throughout the cortex. The

patterns of expression are therefore very different between the NK1, NK2, and NK3 receptors. NK4 receptor mRNA is widely expressed in neurons in the rat CNS, including cerebral cortex, hippocampus, and hypothalamus.49 The NK1 receptor is also coexpressed with nitric oxide synthase in striatal interneurons in the rat.50 In the spinal cord, NK1 receptors are localized Mannose-binding protein-associated serine protease on second-order sensory neurons, receiving nociceptive inputs. The NK1 receptor signal induces a slowly developing sustained depolarization, while the fast input to secondorder sensory neurons is mediated by the excitatory amino acid glutamate through the N-methyl-D-aspartate (NMDA) receptor.37 Specific actions of the neurokinin receptors Elliott and Iversen described the diverse effects of SP after intracerebroventricular (ICV) administration or direct application into the ventral tegmental area of the mesencephalon of rat brain, which caused increased locomotor activity, grooming behavior, and wet dog shakes.51 Repetitive hind paw tapping was also shown to result from activation of central NK1 receptors in gerbils.