2.1] hept-5-en-2-ylmethyl pyrene-1-carboxylate (2). Alternatively, monoliths completely grafted with poly-1 were surface grafted with 2 in a spatially resolved way in the presence of a latent, UV-triggerable precatalyst, i.e., [Ru(IMesH(2))(CF(3)COO)(t-BuCN)(4)(+) CF(3)COO(-)] (5). www.selleckchem.com/products/BAY-73-4506.html Finally, to demonstrate the utility of this chemistry, a 2(nd)-generation Grubbs initiator-based approach was used to prepare a trypsin-functionalized monolith-containing chip device that allowed for the online digestion of N-alpha-benzoyl-L-argininethylester hydrochloride.

(C) 2011 Wiley Periodicals, Inc. J Appl Polym Sci 121: 2551-2558, 2011″
“The licensed UK anthrax vaccine is produced by static cultures of Bacillus anthracis Sterne 34F2 in glass Thompson bottles, each batch consisting of multiple bottles grown for 24-28 h. In this work, a novel miniature bioreactor was used as a scale-down tool to investigate the possible transfer of anthrax vaccine production from static culture to stirred tank operation and to explore the effects of this change in culture conditions on process

performance. It is shown that the change to stirred culture conditions is possible and that the concentration of the two main vaccine components, Protective Antigen (PA) and Lethal Factor (LF), are reached in less than half the time compared to standard Thompson bottle methods. Furthermore, because higher cell densities were attained in the miniature bioreactor, a 74% increase in antigen concentration was achieved. More

detailed analysis of the stirred bioreactor results operated with and without aeration this website showed antigen degradation in the presence of aeration. Overall this work demonstrates the usefulness of miniaturisation techniques to perform process characterisation studies safely and without significant capital investment for large-scale containment. (C) 2010 selleck chemical Elsevier B.V. All rights reserved.”
“Highly branched alkoxysilane (HB) units were prepared in situ via a Michael-type reaction between pentaerythrithol triacrylate and aminopropyltriethoxysilane. These units were used as an inorganic component for the modification of cellulose acetate (CA) films using the sol-gel process. The thermal and dynamic-mechanical behaviors, the morphology, and the dimensional stability of the modified CA films were analyzed. The siloxane-modified CA films showed thermal stability similar to pure CA, but the residue content at 900 degrees C increased with the addition of HB units. The morphology of these films was characterized by siloxane nanodomains dispersed in the CA matrix, with good interfacial adhesion between the phases. Moreover, the CA/siloxane nanocomposite films showed improved dimensional stability in comparison with CA, i.e., in the presence of HB, the dimensional change was reduced to around 50% of the value observed for pure CA.