4%. In comparison, the G+C mol% of the host E. faecalis chromosome is 37.38% (McShan & Shankar, 2002). An analysis of the genome Napabucasin in vivo revealed 65 ORFs. Most initiate translation at an ATG codon (60 ORFs), while GTG (two ORFs) and TTG (three ORFs) are used less frequently. The genome of φEf11 is very condensed in terms of coding sequences: protein-coding sequences account for 92.8% of the genome. The small portion of noncoding sequence is distributed over the genome in an uneven, nonuniform manner. There are 50 bp or fewer in most (43/65

or 66.2%) of the intergenomic, non-protein-coding regions of the genome. The longer (>50 bp) noncoding sequences are distributed among the remaining 22 (33.8%) intergenomic regions, and among these are the most likely candidates for operator/promoter sites. The highly condensed nature of the protein-coding sequences within the genome allows transcriptional read-through, permitting the expression of many genes to be under the control of each operator/promoter.

Therefore, it is to be expected that few regulatory/control sequences would be required within the φEf11 genome. The putative proteins of the entire genome were compared with databases using a web-based manual annotation tool (manatee) at the J. Craig Venter Institute. Protein homologies were identified (Table 1) on the basis of significant blastp matches (P-value ≤10−5 and identity ≥35%) to phage-encoded Compound Library ic50 proteins or similarity Idelalisib chemical structure to proteins with identified functions using BER and HMMs. Of the 65 putative proteins specified in the φEf11 genome, seven (gene products of PHIEF11_006, PHIEF11_0022, PHIEF11_0042, PHIEF11_0043, PHIEF11_0053, PHIEF11_0057, and PHIEF11_0059) showed no matches to any protein from other species, and were termed ‘hypothetical proteins,’ while the majority of the deduced proteins showed significant similarity to proteins in the databases. In most cases, homologies were found

to phages infecting other Gram-positive, low GC bacteria such as Lactococcus lactis, Lactobacillus casei, Streptococcus pyogenes, and E. faecalis (Table 1). A successful bacteriophage infection requires the regulation of gene expression, DNA replication, formation of the phage capsid, and the release of new phage particles from the infected host (Brøndsted et al., 2001). In most bacteriophages, the genes encoding related biological functions are clustered together in functional groups or modules and they are turned on and off in coordination (Ptashne, 2004). The prototype of such a coordinated gene regulation in bacteriophages is the λ family of phages of E. coli (Ptashne, 2004). The genomes of these lambdoid phages typically are composed of 11 modules of clustered, functionally related genes (Casjens et al., 1992).