arjuna extract. The FT-IR results ( Fig. 1) indicated that the functional group obtained for collagen cross-linked T. arjuna bark extract 3439.72 cm−1, 1633.99 cm−1, 1531.04 cm−1, 1448.13 cm−1 did not interfere with functional groups 3401.02 cm−1, 1615.97 cm−1, 1519.53 cm−1, 1448.13 cm−1 of T. arjuna bark extract conforming

their compatibility. The FT-IR results indicated that the functional groups of collagen 1660.86 cm−1-amide I, 1554.77 cm−1-amide II and 1232.62 cm−1-amide III obtained did not interfere with the functional groups SB431542 molecular weight of C. asiatica extract compounds, of 2926 cm−1-Carboxylicacid, 3414 cm−1-hydroxyl, 1691 cm−1-carbonyl, 1485 cm−1 aromatic hydrogen, confirming the extract compatibility. The FT-IR results ( Fig. 2) indicated that the functional group obtained for cross-linked C. asiatica 2959 cm−1, 3371 cm−1, 1640 cm−1, 1443 cm−1

did not interfere with the functional groups 2926 cm−1, 3414 cm−1, 1640 cm−1, 1443 cm−1 of C. asiatica confirming ABT 888 the compatibility. The sterility test conducted on the T. arjuna and C. asiatica extract collagen based films assured the absence of microorganisms. The thickness of the films ( Table 3) was found to be slightly increased with the increase in concentration. The folding endurance results indicated that the TAEICDF’s, TAEICCDF’s, CAEICDF’s & CAEICCDF’s would not break and maintain their integrity till applied to the wounded skin. Equilibrium swelling ratio study results revealed that the scaffolds had a significant impact on the absorption of wound exudates. The higher shrinkage temperature of various extract

incorporated Films suggested increased hydrothermal stability when compared to plain collagen film. The scavenging action of both T. arjuna bark extract & new C. asiatica root extract was well established against peroxy radicals when subjected to time dependence absorbance study. When TAEICDF’s, TAEICCDF’s, CAEICDF’s & CAEICCDF’s were placed on the cellulose paper, sudden decrease in the absorbance value was observed. This might be due to the reaction of C. asiatica root extract, T. arjuna bark extract and collagen with the free radicals preventing them from further peroxidation. The slight increase in the antioxidant efficiency value of TAEICCDF’s & CAEICCDF’s over the TAEICDF’s & CAEICDF’s suggested the more controlled action of the cross-linked films in releasing the extract which gradually increased the antioxidant efficiency. Wound healing studies when performed indicated (Fig. 3) that there was a significant wound healing in the T. arjuna bark extract & C. asiatica root extract treated groups and highest wound healing was observed in the 1.5% TAEICDF’s, 1.5% TAEICCDF’s, 1.5% CAEICDF’s & 1.5% CAEICCDF’s when compared to the wound healing of other groups including the marketed one ( Table 2).