Conclusions Ischemic conditioning seems to prevent HIF-1α mRNA induction in the rat liver after ischemia and reperfusion. This suggests that the protective effects of ischemic

conditioning do not involve the HIF-1 system. On the other hand, the magnitude of the HIF-1α response might be a marker for the degree of I/R injuries after liver ischemia. APR-246 Further studies need to be performed to elucidate this matter. Acknowledgements The excellent technical assistance by Karen Mathiassen and Kirsten Nyborg is highly appreciated. The work was supported by the Health Research Fund of Central Denmark Region, Danish Medical Research Council, the Eva and Henry Frænkels Memorial Foundation and the Clinical Institute, University of Aarhus, Denmark. References 1. Fong Y, Fortner J, Sun RL, Brennan MF, Blumgart LH: Clinical score for predicting recurrence after hepatic resection for metastatic colorectal cancer: analysis of 1001 consecutive cases. AnnSurg 1999, 230:309–318. 2. Abdalla EK, Vauthey JN, Ellis LM, Ellis V, Pollock R, Broglio KR, Hess K, Curley SA: Recurrence and outcomes following hepatic resection, radiofrequency ablation, and combined resection/ablation for colorectal liver metastases. AnnSurg 2004, CP673451 solubility dmso 239:818–825. 3. Pawlik TM, Scoggins CR, Zorzi D, Abdalla EK, Andres A, Eng C, Curley SA, Loyer

EM, Muratore A, Mentha G, et al.: Effect of surgical margin status on survival and site of recurrence after hepatic resection for colorectal metastases. AnnSurg 2005, 241:715–722. discussion 4. Kooby DA, Stockman J, Ben-Porat L, Gonen M, Jarnagin WR, DeMatteo RP, Tuorto S, Wuest D, Blumgart LH, Fong Y: Influence of transfusions on perioperative and long-term outcome in patients following hepatic resection for colorectal metastases. AnnSurg 2003, 237:860–869. 5. Jarnagin WR, Gonen M, Fong Y, DeMatteo RP, Ben-Porat L, Little S, Corvera C, Weber S, Blumgart LH: Improvement in perioperative outcome after hepatic Parvulin resection: analysis of 1,803 consecutive cases over the past decade. AnnSurg 2002, 236:397–406. 6. Rosen CB,

Nagorney DM, Taswell HF, Helgeson SL, Ilstrup DM, van Heerden JA, Adson MA: Perioperative blood transfusion and determinants of survival after liver resection for metastatic colorectal carcinoma. AnnSurg 1992, 216:493–504. 7. van der Bilt JD, Livestro DP, Borren A, van HR, Borel RI: European survey on the application of vascular clamping in liver surgery. Dig Surg 2007, 24:423–435.PubMedCrossRef 8. Delva E, Camus Y, Nordlinger B, Hannoun L, Parc R, Deriaz H, Selumetinib concentration Lienhart A, Huguet C: Vascular occlusions for liver resections. Operative management and tolerance to hepatic ischemia: 142 cases. Ann Surg 1989, 209:211–218. 9. Hannoun L, Borie D, Delva E, Jones D, Vaillant JC, Nordlinger B, Parc R: Liver resection with normothermic ischaemia exceeding 1 h. Br J Surg 1993, 80:1161–1165.PubMedCrossRef 10.