In our study, TLR4 knockdown in vitro lead to TLR4-related inflammatory cytokines being markedly depressed and so it could weaken the ability to the resistance of MDA-MB-231 to CTL and NKC attack and facilitate evasion from immune surveillance. This occurrence in vitro

may indicate us that TLR4 knockdown in vivo could inhibit the growth and promote the death Ro 61-8048 research buy of breast tumors. Conclusions TLR4-mediated cancer growth appears to be an important factor in tumor progression. The use of systemically delivered TLR4-siRNA may provide a novel approach to preventing cancer progression and survival. TLR4AsiRNA directed targeting of TLR4 is a promising candidate for molecular therapy of breast cancer. Acknowledgements This work was supported by Professor Dongxu Liu of Hubei University. References 1. Medzhitov R, Preston-Hurlburt P, Janeway CA Jr: A human homologue of the Drosophila Toll protein signals activation of adaptive immunity. Nature 1997,388(6640):394–397.PubMedCrossRef 2. Takeda K, Kaisho T, Akira S: Toll-like receptors. Annu Rev Immunol 2003, 21:335–376.PubMedCrossRef 3. Medzhitov PSI-7977 datasheet R, Janeway CA Jr: Decoding the patterns of self and nonself by the innate immune system. Science 2002,296(5566):298–300.PubMedCrossRef

4. Takeda K, Akira S: Toll-like Belnacasan nmr receptors in innate immunity. Int Immunol 2005,17(1):1–14.PubMedCrossRef 5. Huang B, Zhao J, Li H, He KL, Chen Y, Chen SH, Mayer L, Unkeless JC, Xiong H: Toll-like receptors on tumor cells facilitate evasion of immune surveillance. Cancer Res 2005,65(12):5009–5014.PubMedCrossRef

6. Droemann D, Albrecht D, Gerdes J, Ulmer AJ, Branscheid D, Vollmer E, Dalhoff K, Zabel P, Goldmann T: Human lung cancer cells express functionally active Toll-like receptor 9. Respir Res 2005,6(1):1–6.PubMedCrossRef 7. Hassan F, Islam S, Tumurkhuu G, Naiki Y, Koide N, either Mori I, Yoshida T, Yokochi T: Inracellular expression of toll-like receptor 4 in neuroblastoma cells and their unresponsiveness to lipopolysaccharide. BMC cancer 2006, 6:281–288.PubMedCrossRef 8. Ilvesaro JM, Merrell MA, Swain TM, Davidson J, Zayzafoon M, Harris KW, Selander KS: Toll like 9 agonists stimulate prostate cancer invasion in vitro. Prostate 2007,67(7):774–781.PubMedCrossRef 9. Molteni M, Marabella D, Orlandi C, Rossetti C: Melanoma cell lines are responsive in vitro to lipopolysaccharide and express TLR4. Cancer Lett 2006,235(1):75–83.PubMedCrossRef 10. Merrell MA, Ilvesaro JM, Lehtonen N, Sorsa T, Gehrs B, Rosenthal E, Chen D, Shackley B, Harris KW, Selander KS: Toll-Like Receptor 9 Agonists Promote Cellular Invasion by Increasing Matrix metalloproteinase activity. Mol Cancer Res 2006,4(7):437–447.PubMedCrossRef 11.