The CC group comprised of 80 females and 127 male participants while SB group of 47 females and 307 male participants. The majority of the subjects were aged between 18 and 30 years of age. Table 1 Percentage and type of dietary supplements used by all participants   Subjects   City centre (207) SC79 Suburbs (354) Supplements use     No 70% 71.2% Yes 30% 28.8% Users of supplements by gender     Male 69.5% 93.1% Female 30.5% 6.9% Frequency of use

    1 time per wk 12.9% 1% 2 time per wk 8.1% 3.9% 3 time per wk 21.0% 32.3% 4 time per wk 17.7% 6.9% 5 time per wk 14.5% 49% 6 time per wk 1.6% 1% 7 time per wk 24.2% 5.9% Palermo, Italy. Frequency distribution Participants provided information of the frequency of weekly consumption of both supplements and foods. Notwithstanding the CC and the SB have broadly the same frequency of buy PF-6463922 protein supplement consumption (30% and 28.8%), weekly use however differs between groups (Table 1).Male gym users demonstrated greater consumption percentages than females. The survey showed that milk is the most frequently consumed food in all groups (68% of CC and 57.8% of SB of the supplement MK-4827 cost users vs. 53% of CC and 63% of SB of non-users) followed by chicken ( 48% in CC and 50% in SB for the supplement users vs. 21% in CC and 28% in SB for non-users)(Figures 1

& 2). Figure 1 Food intake percentage of people who use protein supplements. The figure provides information about the frequency of consumption of gym users who use

protein supplements and their weekly food intake divided in two categories: Greater than 3 times per week and 3 times or lower per week. The data are expressed as percentage. Figure 2 Food intake percentage of people who don’t use protein supplements. The figure provides information about the frequency of consumption of gym users who don’t use protein supplements and their weekly food intake divided in two categories: Greater than 3 times per week and 3 times or lower per week. The data are expressed as percentage. Data also shows that NSU consumed significantly more snacks and bakery products than SU (P < 0.001). Interestingly, the SU consumed significantly higher quantities of vegetables, nuts, fresh fish, eggs clonidine and canned tuna (P < 0.001). Subsequently a comparison between food categories and protein consumption was assessed (Table 2). Table 2 Frequency of food intake stratified by protein content and associated with protein dietary supplements (>3 times per week)   Yes (%) No (%) p     CC SB CC SB   Low content (10 g or below/100 g) Bakery 14.5 24.5 18.6 43.7     Milk 67.7 57.8 52.4 63.1 < 0.01   Snack 11.3 21.6 26.2 10.7     Yogurt 41.9 25.5 24.8 29     Mean% 33.85 32.35 29.75 36.6   Medium content (10-20 g/100 g) Legumes 29 16.7 9 19     Nuts 11.3 22.5 2.8 15.9     Cheese 32.2 23.5 28.3 9.9 ns   Mean% 24.2 20.9 13.4 14.9   High content (20-25 g or above/100 g) Meat 33.9 24.5 33.8 14.3     Eggs 24.1 24.5 3.4 6.3     Fresh Fish 22.5 7.8 10.3 4.4 < 0.