The sequences of the four 16S rRNA gene copies in the genome do not differ from each other, and do not differ from the previously published 16S rRNA gene sequence (“type”:”entrez-nucleotide”,”attrs”:”text”:”AM943630″,”term_id”:”171853308″,”term_text”:”AM943630″AM943630), http://www.selleckchem.com/products/Vorinostat-saha.html which contains two ambiguous base calls. Figure 1 Phylogenetic tree highlighting the position of P. caeruleus relative to the type strains of the other species within the genus Phaeobacter and the neighboring genera Leisingera and Oceanicola [4-17]. The tree was inferred from 1,387 aligned characters … A representative genomic 16S rRNA gene sequence of P. caeruleus 13T was compared using NCBI BLAST [25,26] under default settings (e.g.

, considering only the high-scoring segment pairs (HSPs) from the best 250 hits) with the most recent release of the Greengenes database [27] and the relative frequencies of taxa and keywords (reduced to their stem [28]) were determined, weighted by BLAST scores [Table 1]. The most frequently occurring genera were Phaeobacter (38.5%), Ruegeria (18.6%), Roseobacter (15.0%), Silicibacter (11.9%) and Leisingera (5.5%) (74 hits in total). Regarding the single hit to sequences from members of the species, the average identity within HSPs was 100.0%, whereas the average coverage by HSPs was 96.9%. Regarding the nine hits to sequences from other members of the genus, the average identity within HSPs was 97.6%, whereas the average coverage by HSPs was 99.5%.

Among all other species, the one yielding the highest score was Phaeobacter gallaeciensis (“type”:”entrez-nucleotide”,”attrs”:”text”:”AY881240″,”term_id”:”62183719″,”term_text”:”AY881240″AY881240), which corresponded to an identity of 98.3% and an HSP coverage of 99.3%. (Note that the Greengenes database uses the INSDC (= EMBL/NCBI/DDBJ) annotation, which is not an authoritative source for nomenclature or classification.) The highest-scoring environmental sequence was “type”:”entrez-nucleotide”,”attrs”:”text”:”EF573869″,”term_id”:”148731650″,”term_text”:”EF573869″EF573869 (Greengenes short name ‘site S25 near Coco’s Island marine clone S25 213′), which showed an identity of 98.8% and an HSP coverage of 99.9%. The most frequently occurring keywords within the labels of all environmental samples which yielded hits were ‘coral’ (6.8%), ‘caribbean’ (5.8%), ‘faveolata’ (5.5%), ‘chang’ (5.

4%) and ‘disease-induc, montastraea, plagu, white’ Entinostat (5.2%) (169 hits in total). Environmental samples which yielded hits of a higher score than the highest scoring species were not found, indicating that the species is rarely found in environmental samples. Table 1 Classification and general features of P. caeruleus DSM 24564T according to the MIGS recommendations [29]. Morphology and physiology P. caeruleus 13T cells are Gram-negative rods with a cell size of 0.9-1.8 ��m (Figure 2).