We aimed to detect the expression of SSTR2 and SSTR5 in GEP-NEN and their significance in predicting clinical behaviors and outcomes. Methods: We examined immunohistochemicaly the SSTR2 and SSTR5 protein in 112 specimens with GEP-NEN from November 1995

to December 2012 in The First Affiliated Hospital, Sun Yat-sen University. Results: The overall expression rates of SSTR2 and SSTR5 were 64.3% and 53.6%, respectively. The positive SSTR2 was associated with tumors located in pancreas, classified as G1 and NET (P < 0.5), tumors without distant metastasis also expressed higher SSTR2 than that Ponatinib chemical structure with metastasis (70.5% vs. 50.0%, P = 0.037). The positive SSTR5 was associated with G1 tumors and NET (P < 0.5). The

co-expression rate of SSTR2 and SSTR2 was 47.3%, the expression of SSTR2 had positive correlation with that of SSTR5 (r = 0.539, P = 0.000). In survival analysis, patients with SSTR2 positive showed better prognosis than negative ones (χ2 = 3.826, Alvelestat P = 0.05), and these expression of SSTR5 patients were not correlated with prognosis (χ2 = 2.585, P = 0.108). Conclusion: SSTR2 and SSTR5 are widely expressed in the NET, G1. The positive expression of SSTR2 was related to relatively benign clinical behaviors and better prognosis in GEP-NEN. Key Word(s): 1. gep; 2. neuroendocrine; 3. neoplasm; 4. sstr; Presenting Author: CHUN-HUA QIE Corresponding Author: CHUN-HUA QIE Affiliations: Tianjin Second People’s Hospital Objective: we established a Hyper-IL-6-expressing

mouse hepatocellular carcinoma cell clones using gene transfection and observed the antitumor effect of the cancer vaccine. selleck chemicals Methods: The recombinant plasmid was transfected into mouse hepatocellular carcinoma cells MM45T. Li by lipofectamine method and positive cell clones were obtained by G418 resistant screening and limiting dilution. The expression of Hyper-IL-6 in the transfected cells was confirmed with RT-PCR analyses. In vitro experiment we measured the proliferation capability by MTT assays. In vivo experiment were performed to observe the tumorigenicity of wild type MM45T. Li and Hyper-IL-6 gene transfected mouse hepatocellular carcinoma cells. Results: Expression of Hyper-IL-6 gene was identified with RT-PCR analyses in the transfected MM45T. Li cells, but not in the wild type MM45T. Li. In vitro experiment the proliferation capability of Hyper-IL-6 gene transfected MM45T. Li cells has not obviously altered compared with the wild type MM45T. Li.