When the tumor size became 3 mm in diameter, the control group was injected with PBS solution with 5% dextrose, while the experimental group was intraperitoneally injected with 100 ��l of KML001 at a concentration of 10 mg/kg with 5% dextrose. At 8, www.selleckchem.com/products/Axitinib.html 24 and 48 hours after injection, the liver, spleen and tumor tissues were acquired and put into a 37% solution of formaldehyde for 24 hours. The tissues were inserted into paraffin and sectioned at a thickness of 4 ��m using a microtome (SLEE MAINZ GmbH, Germany). The sections were placed on slides and stained with Mayer’s hematoxylin and eosin Y (both from Sigma-Aldrich). Images were observed using a model BX50 inverted microscope (Olympus, Tokyo, Japan). Tumor Perfusion Measurement Magnetic resonance imaging (MRI) examination was performed 8 and 9 days after subcutaneous inoculation of 2��106 CT26 cells.

Balb/C mice were anesthetized by 2% isoflurane mixed with 100% 1 l/min O2 via a nose cone. A catheter was inserted into the tail vein for injection of 281 mg/kg gadopentetic acid-diethylene triaminepentaacetic acid (Gd-DTPA; Magnevist, Schering, Germany). T2-weighted turbo spin echo images were acquired on coronal planes using the following parameters: TE=60 ms, TR=3200 ms, field of view (FOV)=50 mm, RFOV (%)=69.94%, matrix scan=224, reconstruction=512, 0.5 mm thick slices, 14 slices, and acquisition time=8 min 38 s. T1-weighted spin-echo images were acquired on coronal planes as pre- and post-dynamic contrast enhanced MRI scan using the following parameters: TE=7.

9 ms, TR=427 ms, field of view (FOV)=50 mm, RFOV (%)=70%, matrix scan=128, reconstruction=256, 1 mm thick slices, 11 slices, and acquisition time=2 min 8 s. For dynamic contrast enhanced (DCE)-MRI scan, Gd-DTPA (Magnevist) was administrated at a dose of 281 mg/kg via tail-vein injection. DCE-MRI was performed using a perfusion-weighted spin echo sequence using the following acquisition parameters: FOV (mm)=50��35, RFOV (%)=71, matrix scan=112, reconstruction=224, TR/TE (ms)=12/4.0, slice number=11, dynamic scans=60, 1 mm slice thickness with a total acquisition time of 2 min 6 s. MRI images were downloaded from a Philips MRI scanner in the Philips PARREC format, and the images were transferred to a processing workstation connected to the research network. The programming languages used were IDL 8.1 (ITT, Boulder, CO, USA).

The data was analyzed by the BRIX model method. Cytotoxicity Assay The cytotoxicity assay was used to determine drug-mediated cytotoxicity Carfilzomib by using (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) reagent of the Cell Titer 96 Aqueous One Solution (Promega, Madison, WI, USA) with absorption measured at 490 nm, as described previously [16]. Briefly, the assay entailed seeding HUVECs at 0.5��105 cells per well for 24 h at 37��C and 5% CO2.