The present results strengthen the possibility that celecoxib has also mechanisms of analgesia unrelated to COX inhibition but dependent on endogenous opioid release. Our results also imply the existence of a new class of analgesics without the deleterious effects of COX inhibitors. (C) 2010 Elsevier Ltd. All rights reserved.”
“The wound healing is a complex process consisting of inflammatory reaction, proliferation of mesenchymal cells, and formation and contraction of granulation tissue. The integrin receptors have crucial roles in this process. Recently, alpha 9 integrin has also been detected on keratinocytes
within wound Luminespib sites. However, its functional significance at various wound healing processes was not fully elucidated. To address the role of alpha 9 integrin in wound healing process, we made a full-thickness skin excisional wound and treated mice with anti-alpha 9 integrin antibody. It has been shown that wound healing process was divided into three distinct phases: first, the re-epithelialization phase, second, the phase of granulation tissue formation, and finally the phase of contraction of granulation tissue. We LY2835219 manufacturer found that contraction of granulation tissue was not impaired by blocking the interaction of alpha 9 integrin with its ligands, indicating
that alpha 9 integrin is not involved in myofibroblast differentiation. It is noteworthy that the formation of granulation tissue, as characterized by dense vimentin and CD31-positive area, was impaired. The hindrance of granulation tissue formation is because of the inhibition of adhesion and migration of alpha 9 integrin-positive dermal fibroblasts. In conclusion, alpha 9 integrin is involved in the formation of granulation tissue through regulating migration and adhesion of
dermal Rolziracetam fibroblasts in the full-thickness skin excisional wound model. Laboratory Investigation (2010) 90, 881-894; doi:10.1038/labinvest.2010.69; published online 22 March 2010″
“Inhibitors of the glycine transporter GlyT-1 are being developed as potential treatments for schizophrenia. Here we report on the use of two novel radioligands, [(3)H]-SB-733993 and [(3)H]-GSK931145, for the characterisation of GlyT-1 in both cells and native tissue. Binding was evaluated in membranes either from HEK293 cells expressing recombinant human GlyT-1 (hGlyT-1) or from rat cerebral cortex. Specific binding of both [(3)H]-SB-733993 and [(3)H]-GSK931145 to hGlyT-1 HEK293 cell membranes and rat cerebral cortex membranes was saturable and comprised >90% of total binding. IQ and B(max) values for the two radioligands were fairly similar, with K(d) values of 1-2 nM and B(max) values of around 7000 fmol/mg protein in hGlyT-1 membranes and 3000 fmol/mg protein in rat cortex membranes. Association of [(3)H]-SB-733993 was faster, with binding reaching equilibrium within 30 min compared with 90 min for [(3)H]-GSK931145.