Then proliferation of LoVo cells was determined by methyl thiazol tetrazolium (MTT) assay, and observed the changes of cell morphology by inverted microscope. The activities
of motility and invasion of LoVo cells were assessed by transwell chamber inwasion assay in vitro. Flow cytometry was used for cell cycle analysis. Reverse transcription-polymerase chain reaction (RT-PCR) was used to semi-quantify the RIP1 mRNA level. Results: The abilities of proliferation were inhibited (0.560 ± 0.023 vs 0.930 ± 0.034, P < 0.05), and typical apoptosis cellular morphology of LoVo cells was observed under the inverted microscope, but the nonspecific RIP1 siRNA and the blank control group had no effect on LoVo learn more cells. The motility and inwation of LoVo cells were inhibited significantly (21 ± 2.731 vs 43 ± 2.064), and the colorectal cancer cells penetrated polycarbonate membrane notable reduction. The nonspecific siRNA and the blank control group had no effect
on LoVo cells. The number of cells in G0∼G1 phrase increased in RIP1 siRNA group 58.28% selleck products in comparison with negative control 48.88% and blank control groups 43.82%. The nonspecific RIP1 siRNA and the blank control group had no effect on LoVo cells. In the RIP1 siRNA group, the RIP1 mRNA level was down-regulaed remarkably (P < 0.05). Conclusion: After the transfection of the RIP1-targeted siRNA into LoVo cells, the expression of RIP1 gene has been knockoutted effectively. Silencing RIP1 could regulae the malignant biological behavrors of colon cancer cell line LoVo effectively. The abilities NADPH-cytochrome-c2 reductase of proliferation and the motility and inwation of LoVo cells
were inhibited. The results shown that RIP1 gene played the important role of the proliferation and apoptosis regulation in colorectal carcinoma cells. Key Word(s): 1. RNA interference; 2. RIP1 gene; 3. Colorectal carcinoma; Presenting Author: YU-RONG WENG Additional Authors: YA-NAN YU, LIN-LIN REN, YUN CUI, YOU-YONG LV, WEIBIAO CAO, JIE HONG, JING-YUAN FANG Corresponding Author: JIE HONG, JING-YUAN FANG Affiliations: Renji Hospital, Shanghai Jiao-Tong University School of Medicine; Peking University; The Warren Alpert Medical School of Brown University & Rhode Island Hospital Objective: C9orf140 is a newly identified and characterized gene which is associated with cell proliferation and tumorigenicity. Expression of C9orf140 is upregulated in human gastric cancer and colorectal cancer (CRC); however, little is known about its role in CRC invasion. Methods: We have investigated the clinical significance, biological effects and mechanisms of C9orf140 signaling. Results: Our finding showed that knockdown of C9orf140 significantly reduced cell invasion in vitro and dramatically increased overall survival and decreased lung metastasis in vivo.