Results: At the end of the study evidence of urothelial hyperplasia was seen in 50% of the pigs in group 1 and in 29% in group 2. Four and 2 cases of cranial stent migration in groups 1 and 2, respectively, were seen at 6 months. Hyperplasia and renal involvement were statistically significantly different between the groups with more damage in group 1 than in group 2.
Conclusions: Hyperplasia was markedly reduced when ureteral peristalsis was inhibited by endoureterotomy
at the area of interaction between the stent and the ureter.”
“Purpose: We investigated Sapanisertib whether analysis of adherence junctions in human detrusor could be used as a diagnostic tool to determine detrusor overactivity.
Materials and Methods: We characterized the protein composition of adherence junctions in the human bladder using cadherin-11 since our group previously found that cadherin-11 could be an integral structural protein of adherence junctions. We obtained a total of 46 biopsies from 23 patients categorized into 4 groups, including 5 who were normal, and 6 each with neurogenic disease with detrusor overactivity, bladder outlet obstruction with detrusor overactivity and idiopathic detrusor overactivity. Specimens were processed to study cadherin-11 expression using combined immunohistochemical and immunogold electron microscopy techniques.
Cadherin-11 expression was semiquantitatively analyzed and correlated to muscle PD173074 research buy fascicle structure and collagen in the extracellular spaces.
Results: Immunogold labeling showed highly specific cadherin-11 expression at adherence junctions in detrusor smooth muscle cells. During immunohistochemical staining a wide variety of cadherin-11 expression and fascicle structure was found in the same specimen. No correlation was noted between detrusor overactivity and cadherin-11 expression. However, cadherin-11
seemed to be down-regulated with intercellular space widening and collagenosis.
Conclusions: Cadherin-11 is an integral structural protein of the adherence junction. Defects in the overactive detrusor are highly punctate. Quantitative Branched chain aminotransferase analysis of adherence junctions using biopsy cannot replace urodynamic evaluation as a predictor of detrusor overactivity in the human bladder.”
“Purpose: Bladder problems clinically present early in life as birth defects that often lead to kidney failure and late in life as overactive bladder, incontinence and related disorders. We investigated the transcriptome of mouse bladder mucosa at juvenile and adult stages by microarray to identify the pathways associated with normal, healthy growth and maturation. We hypothesized that understanding these pathways could be key to achieving bladder regeneration or reawakening normal function in the elderly population.
Materials and Methods: RNA was isolated from the mucosa at 3, 6, 20 and 30 weeks postnatally. Affymetrix (R) Mouse 430 v2 arrays were used to profile the expression of approximately 45,000 genes.